Updated 16S rRNA-RFLP method for the identification of all currently characterised Arcobacter spp
نویسندگان
چکیده
منابع مشابه
Extended method for discrimination of Aeromonas spp. by 16S rDNA RFLP analysis.
A previously described molecular method, based on 16S rDNA RFLP analysis, for the identification of Aeromonas spp. was unable to separate the species Aeromonas salmonicida, Aeromonas bestiarum and the recently described Aeromonas popoffii. In this study, the method has been extended with endonucleases AIwNI and PstI for the identification of these species. A molecular frame for the identificati...
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Background: There is a conserved portion in the 16S rRNA gene of bacteria which can be amplified by the universal PCR method. This fragment is 996 bp in length. In this method, only one set of universal primers is used for the amplification of the conserved region of the 16S rRNA gene, in common bacterial pathogens. Therefore, using the universal PCR method, these bacteria are detectable on...
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A rapid two-step identification scheme based on PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the 16S rRNA gene was developed in order to differentiate isolates belonging to the Campylobacter, Arcobacter, and Helicobacter genera. For 158 isolates (26 reference cultures and 132 clinical isolates), specific RFLP patterns were obtained and species were successfully identified...
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API 20E strip test, the standard for Enterobacteriaceae identification, is not sufficient to discriminate some Yersinia species for some unstable biochemical reactions and the same biochemical profile presented in some species, e.g. Yersinia ferderiksenii and Yersinia intermedia, which need a variety of molecular biology methods as auxiliaries for identification. The 16S rRNA gene is considered...
متن کاملMolecular Identification of the Persian Gulf Sea Hare (Aplysia sp.) Based on 16s rRNA Gene Sequence
Background: Sea hares of the Aplysia genus are among the mollusks of interest for various researchers to study their phylogeny, bioactive compounds and the nervous system. These mollusks are herbivorous and produce chemical compounds (ink) to defend themselves. The present study provided molecular identification of the Persian Gulf (Bushehr city) sea hare using 16s rRNA gene sequence. Materials...
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ژورنال
عنوان ژورنال: BMC Microbiology
سال: 2012
ISSN: 1471-2180
DOI: 10.1186/1471-2180-12-292